Transcriptomic Profiling of the Arabidopsis Embryonic Epidermis Using FANS in Combination with RNAseq
| Autor: | Janika Witthöft, Daniel Slane, Kenneth W. Berendzen, Gerd Jürgens |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Messenger RNA Cell RNA RNA-Seq Meristem Biology biology.organism_classification Embryonic stem cell Cell biology Transcriptome 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure Arabidopsis medicine 030217 neurology & neurosurgery 030304 developmental biology |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071603413 |
| DOI: | 10.1007/978-1-0716-0342-0_12 |
| Popis: | The fundamental mechanisms of cell identity and tissue establishment are important already from the very beginning of a plant's life and reiterate later during development. In order to unravel and understand the underlying mechanisms to generate differences that in turn lead to cell or tissue types, plant cells have to be separated and their transcriptional setup analyzed. We have previously demonstrated that fluorescence-activated nuclear sorting (FANS) is a powerful tool to generate nuclear transcriptomic profiles of the most inaccessible embryonic tissues. In this protocol, we extend this effort to combine FANS with next generation RNA sequencing (RNA-seq) to achieve early embryonic transcriptomes of Arabidopsis epidermis precursor tissue (protoderm) and the inner tissue counterpart. |
| Databáze: | OpenAIRE |
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