Carboxyhemoglobin determination by second-derivative spectroscopy

Autor:	J. Parks, H. G. J. Worth
Rok vydání:	1985
Předmět:	chemistry.chemical_compound chemistry Biochemistry (medical) Clinical Biochemistry Carboxyhemoglobin Analytical chemistry Second derivative spectroscopy Oxidation reduction Hemoglobin Spectrum analysis Spectral shift Reduced hemoglobin Biological fluid
Zdroj:	Clinical Chemistry. 31:279-281
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/31.2.279
Popis:	In this procedure hemoglobin is converted to its reduced form and the magnitude of the zero-order spectral shift of the reduced hemoglobin peak at 430 nm to the carboxyhemoglobin peak at 418 nm is determined by second-derivative spectrum analysis. The method is simple, straightforward to set up, and rapid. A result may be obtained within 15 min of receiving the sample. It is sufficiently sensitive to differentiate carboxyhemoglobin concentration in the blood of smokers and nonsmokers.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::df2c7d557c37d9f99abdd05750c9df9d https://doi.org/10.1093/clinchem/31.2.279 Zobrazit plný text záznamu