Simultaneous Quantification of Piperine, Vasicine and Eugenol in Kabasura Kudineer by HPTLC

Autor: M. K. Mohan Maruga Raja, null Drashti C. Patel
Rok vydání: 2023
Předmět:
Zdroj: Journal of Natural Remedies. :231-235
ISSN: 2320-3358
0972-5547
DOI: 10.18311/jnr/2023/31918
Popis: Introduction: Kabasura kudineer is a polyherbal decoction of Siddha medical system (an Indian system of medicine), traditionally used to cure fever, colds, coughs, and respiratory ailments. Government of India had recommended kabasura kudineer as one among many preventive/treatment measures for COVID-19. Kabasura kudineer choornam is an admixed coarse powder of 15 herbs and its decoction is kabasura kudineer. The major chemical constituents present in the 15 herbs required to prepare the choornam are known but the chemical composition of the prepared kabasura kudineer (decoction) are unknown. Objective: As a part of the chemical investigation, piperine, vasicine and eugenol were chosen as marker compounds in kabasura kudineer based on their literature for exhibiting potent activity against upper and lower respiratory tract infections. The current work was planned to identify and quantify piperine, vasicine and eugenol simultaneously by TLC in kabasura kudineer. Methods: Silica gel 60F254 TLC plates were used for the study. The optimized mobile phase used was toluene: ethyl acetate: methanol: ammonia (5:9:3:0.5, v/v/v/v) and the densiometric scanning was done at 287 nm. Results: The Rf values of piperine, vasicine and eugenol were found to be 0.70, 0.32 and 0.82 respectively. Linearity range of piperine and vasicine was 500- 3000 ng spot-1 and it was 10- 60 ng spot-1 for eugenol. The amount of piperine, vasicine and eugenol in 100 mL of kabasura kudineer was found to be 0.03, 0.056 and 0.035% w/v respectively. The developed method was validated as per ICH guideline parameters. Conclusion: The current study shows that the developed TLC method is simple, precise, specific, robust and accurate. This method will also be useful to identify or quantity any polyherbal formulation containing piperine, vasicine and eugenol as chemical constituents.
Databáze: OpenAIRE
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