QuEChERS-HPLC METHOD FOR AFLATOXIN DETECTION OF DOMESTIC AND IMPORTED FOOD IN JORDAN

Autor:	Ahmad F Al-Shunnaq, Richard C.S. Wong, Ala’ Yahya Sirhan, Lukman Bola Abdulra’uf, Guan Huat Tan
Rok vydání:	2013
Předmět:	Aflatoxin Chromatography Clinical Biochemistry Extraction (chemistry) Pharmaceutical Science Quechers Biochemistry High-performance liquid chromatography Analytical Chemistry Solvent chemistry.chemical_compound chemistry Methanol Mycotoxin Hplc method
Zdroj:	Journal of Liquid Chromatography & Related Technologies. 37:321-342
ISSN:	1520-572X 1082-6076
Popis:	A method for the analysis of aflatoxins in food samples was developed. The preliminary work focused on optimizing sample pretreatment conditions such as the extraction solvent; the type and amount of drying agents; the extraction time; and the solvent-sample ratio. A 60:40 methanol/acetonitrile (%, v/v) extraction mixture provided recoveries in the range of 71.8–107.7% at spike levels of 1.5–50.0 µg/kg with relative standard deviations (RSDs) lower than 15%. The separation of the four aflatoxin compounds with high selectivity and sensitivity, and within reasonable retention times, was performed by a C18 250 mm × 4.6 mm × 5 µm column (Nucleodur 250-5) at 45°C. The developed QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) procedure, high performance liquid chromatography (HPLC) method was then compared with the fluorometric determination method. It was found that the fluorometric determination method had a poor precision and positive bias as compared to QuEChERS-HPLC method. A total of 669 domest...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::df0b4423074f83ef5468fcd0f2f5e52e https://doi.org/10.1080/10826076.2012.745138 Zobrazit plný text záznamu