Ultrasonic-assisted extraction optimized by response surface methodology, chemical composition and antioxidant activity of polysaccharides from Tremella mesenterica
| Autor: | Chen-huan Yu, Xiao-xian Li, Jing Chen, Yun-liang Yan, Shi-qing Li, Wei Wang |
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| Rok vydání: | 2011 |
| Předmět: |
chemistry.chemical_classification
Chromatography Polymers and Plastics biology Organic Chemistry Extraction (chemistry) Raw material Xylose Polysaccharide biology.organism_classification chemistry.chemical_compound Biochemistry chemistry Yield (chemistry) Materials Chemistry Response surface methodology Hydrogen peroxide Tremella mesenterica |
| Zdroj: | Carbohydrate Polymers. 83:217-224 |
| ISSN: | 0144-8617 |
| DOI: | 10.1016/j.carbpol.2010.07.045 |
| Popis: | Box–Behnken design was employed to optimize the ratio of water to raw material (X1: 20–40 mL/g), extraction time (X2: 20–40 min) and extraction temperature (X3: 40–60 °C) to obtain a high crude polysaccharides yield from Tremella mesenterica (TMP) by ultrasonic-assisted extraction technique (UAE). The optimum conditions were a ratio of water to raw material of 34 mL/g and extraction time of 30 min at 50 °C. Under these conditions, the experimental yield was 8.26%, well matched with the predicted models with the coefficients of determination (R2) of 0.9918. The crude TMP was purified by Sephadex G-100 chromatography, giving two major fractions (TMPA and TMPB), which were composed of xylose, mannose and glucose in molar ratio of 1.00:4.74:0.91 and 1.00:6.63:2.34, respectively. The evaluation of antioxidant activity suggested that the polysaccharides exhibited significant protection against hydrogen peroxide induced oxidative damage in rat erythrocytes and could be explored as a nutraceutical agent. |
| Databáze: | OpenAIRE |
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