| Popis: |
Selenium (Se) is an interesting element for bioanalytical chemists. Se forms Se-containing compounds having Se-carbon covalent bond(s), i.e., selenometabolites in its metabolic pathway. In this chapter, the analytical techniques for the speciation and identification of unique selenometabolites in animals are highlighted. First, the instruments required for analyses are overviewed. In particular, hyphenated techniques consisting of high-performance liquid chromatography and inductively coupled plasma (tandem) mass spectrometry (ICP-MS) or electrospray ionization (tandem) mass spectrometry are focused on. Second, laser ablation hyphenated with ICP-MS for Se imaging is briefly overviewed. Then, advanced techniques of nuclear magnetic resonance (NMR) spectroscopy for Se analysis are mentioned with an application to a biological sample. |
