Immobilization engineering – How to design advanced sol–gel systems for biocatalysis?
| Autor: | Sándor Kemény, Márk Oláh, László Poppe, György Marosi, Diána Weiser, Ákos Gellért, András Szilágyi, Gergely Bánóczi, Attila Farkas, Flóra Nagy, Krisztina László |
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| Rok vydání: | 2017 |
| Předmět: |
biology
010405 organic chemistry Chemistry Industrial scale Substrate (chemistry) Lipase b 02 engineering and technology Flow chemistry Enzyme entrapment 021001 nanoscience & nanotechnology biology.organism_classification 01 natural sciences Pollution 0104 chemical sciences Chemical engineering Biocatalysis Environmental Chemistry Organic chemistry Candida antarctica 0210 nano-technology Sol-gel |
| Zdroj: | Green Chemistry. 19:3927-3937 |
| ISSN: | 1463-9270 1463-9262 |
| DOI: | 10.1039/c7gc00896a |
| Popis: | An immobilization engineering approach using bioinformatics and experimental design tools was applied to improve the sol–gel enzyme entrapment methodology. This strategy was used for the immobilization of lipase B from Candida antarctica (CaLB), a versatile enzyme widely used even on the industrial scale. The optimized entrapment of CaLB in sol–gel matrices is reported by the response-surface methodology enabling efficient process development. The immobilized CaLBs characterized by functional efficiency and enhanced recovery provided economical and green options for flow chemistry. Various ternary mixtures of sol–gel precursors allowed the creation of tailored entrapment matrices best suited for the enzyme and its targeted substrate. The sol–gel-entrapped forms of CaLB were excellent biocatalysts in the kinetic resolutions of secondary alcohols and secondary amines with aromatic or aliphatic substituents both in batch and continuous-flow biotransformations. |
| Databáze: | OpenAIRE |
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