Effect of Cytokinins on the Micropropagation of Carob (Ceratonia siliqua L.) through Shoot Tip Culture

Autor:	Zineb Nejjar El Ansari, Safaâ Rahmouni, Ahmed Lamarti, Alain Badoc, Rabah Saidi, Abdelfettah Maouni
Rok vydání:	2019
Předmět:	Organogenesis General Medicine food.food Horticulture chemistry.chemical_compound Ceratonia siliqua food chemistry Micropropagation Cytokinin Shoot Kinetin Zeatin Explant culture
Zdroj:	American Journal of Plant Sciences. 10:1469-1481
ISSN:	2158-2750 2158-2742
Popis:	In order to determine the most suitable cytokinin for the micropropagation of carob (Ceratonia siliqua L.), the effect of four cytokinins: BAP, zeatin, kinetin and 2-iP, was tested on explants derived from young seedlings of seven days. Organogenesis is better in the presence of BAP (0.5 mg/l), while buds growth is favored by zeatin (0.5 mg/l). The combination of the most suitable cytokinin (BAP at 0.5 mg/l) with IBA improves the caulogenesis when the concentration of this latter is low (0.1 mg/l); high concentrations of IBA have an inhibitory effect on elongation and neoformation of shoots and leaves. The multiplication and growth of shoots are more favorable on WPM medium in the presence of BAP (0.5 and 1 mg/l) alone or combined with 0.5 mg/l with GA3, while rooting is mainly favored by IBA, especially at 2 mg/l.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::def766c41d2cdfe6d41b10e191f47024 https://doi.org/10.4236/ajps.2019.109104 Zobrazit plný text záznamu