Effect of Cytokinins on the Micropropagation of Carob (Ceratonia siliqua L.) through Shoot Tip Culture
Autor: | Zineb Nejjar El Ansari, Safaâ Rahmouni, Ahmed Lamarti, Alain Badoc, Rabah Saidi, Abdelfettah Maouni |
---|---|
Rok vydání: | 2019 |
Předmět: | |
Zdroj: | American Journal of Plant Sciences. 10:1469-1481 |
ISSN: | 2158-2750 2158-2742 |
Popis: | In order to determine the most suitable cytokinin for the micropropagation of carob (Ceratonia siliqua L.), the effect of four cytokinins: BAP, zeatin, kinetin and 2-iP, was tested on explants derived from young seedlings of seven days. Organogenesis is better in the presence of BAP (0.5 mg/l), while buds growth is favored by zeatin (0.5 mg/l). The combination of the most suitable cytokinin (BAP at 0.5 mg/l) with IBA improves the caulogenesis when the concentration of this latter is low (0.1 mg/l); high concentrations of IBA have an inhibitory effect on elongation and neoformation of shoots and leaves. The multiplication and growth of shoots are more favorable on WPM medium in the presence of BAP (0.5 and 1 mg/l) alone or combined with 0.5 mg/l with GA3, while rooting is mainly favored by IBA, especially at 2 mg/l. |
Databáze: | OpenAIRE |
Externí odkaz: |