| Popis: |
Fritillariae cirrhosae bulbus (BM CPMs), which is used as an effective treatment for moistening lung, has been listed in the third level of protection category due to its decline in the wild. The demand has greatly increased since 2013 along with the growing concern over the high frequency of fog and haze. Shedanchuanbei particles are one of the adulterants or substituents that frequently occur in Chinese patent medicines containing BM CPMs. In this research, we first established a BM database comprising 208 ITS2 sequences from 25 species of Fritillaria and 1 adulterant. We then collected 20 commercial BM CPMs, selecting 20 CPMs for cloning experiments, which included 15 batches of shedanchuanbei particles, 4 batches of chuanbeimo particles, and 1 batch of shedanchuanbei powder. Results of cloning experiments revealed that among 15 batches of shedanchuanbei particles, 3 batches contained F. ussuriensis bulbus, 10 batches contained F. pallidiflorae bulbus, and 14 batches contained F. verticillate . Moreover, among 4 batches of chuanbeimo particles, only 2 batches contained BM CPM, but these batches were adulterated with F. ussuriensis bulbus and F. verticillate. Both F. pallidiflorae bulbus and F. verticillate were detected in shedanchuanbei powder. Three batches of shedanchuanbei particles were also analyzed using high-throughput sequencing, which detected no BM CPM. This study presents the seriousness of the herbal adulterant problem and the feasibility of the TA cloning with high-throughput sequencing for identifying CPM. |
