| Autor: |
Jens Steinbrenner, Daniel F. A. Tomé, Jim Beynon |
| Rok vydání: |
2014 |
| Předmět: |
|
| Zdroj: |
Methods in Molecular Biology ISBN: 9781627039857 |
| DOI: |
10.1007/978-1-62703-986-4_12 |
| Popis: |
There is a considerable interest in determining the role of individual oomycete effectors in promoting disease. Widely used strategies are based on manipulating effector-expression levels in the pathogen and by over-expressing particular effectors in the host by genetic transformation. In the case of the oomycete, Hyaloperonospora arabidopsidis (Hpa) genetic manipulation is not yet possible, so over-expression of predicted effectors in stably transformed Arabidopsis lines is used to investigate their capability for promoting virulence. Here, we describe a technique for quantifying pathogen growth based on the counting of asexual reproductive structures called sporangiophores in the compatible interaction between the Hpa isolate Noks1 and the Col-0 Arabidopsis accession. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
