First application of PCR-Luminex system for molecular diagnosis of fungicide resistance and species identification of fungal pathogens
| Autor: | Junichiro Yamaguchi, Toshitaka Iwama, Hideo Ishii, Motoshige Shimizu, Junko Tanoue, Wen-Hsin Chung, Michiyo Oshima, Hideaki Yoshimatsu, Kazuhiro So, Kumiko Nishimura, Toru Kozawa, Fumihiro Nemoto |
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| Rok vydání: | 2008 |
| Předmět: | |
| Zdroj: | Journal of General Plant Pathology. 74:409-416 |
| ISSN: | 1610-739X 1345-2630 |
| DOI: | 10.1007/s10327-008-0119-z |
| Popis: | Fungicide resistance in plant pathogens is often caused by a single point mutation in a gene encoding fungicide target proteins. Such is the case for resistance to MBI-D (inhibitors of scytalone dehydratase in melanin biosynthesis) fungicides in rice blast fungus (Magnaporthe oryzae), which is caused by a mutation in the scytalone dehydratase gene that results in a replacement of valine with methionine at codon 75 of the fungicide target protein. PCR-Luminex, a novel system developed for high-throughput analysis of single nucleotide polymorphisms (SNPs) was successfully introduced to diagnose MBI-D resistance using specific oligonucleotide probes coupled with fluorescent beads. The PCR-Luminex system was further tested for its potential in identifying species causing Fusarium head blight on wheat. Four major pathogens, Fusarium graminearum (=F. asiaticum), F. culmorum, F. avenaceum, and Microdochium nivale, known to cause the disease, were tested, and the species were identified using the PCR-Luminex method. So far, this report is the first on the application of the DNA-based PCR-Luminex system in the area of crop protection and/or agricultural sciences. |
| Databáze: | OpenAIRE |
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