| Popis: |
The specific molecular differences between the edematous and necrotizing form of pancreatitis are mainly unknown. We performed a study to investigate genomic expression in rat pancreas in early time course of both edematous and necrotizing pancreatitis. In male wistar rats (10 groups, 4 animals/ group) we induced edematous pancreatitis (CIP) by two consecutive i.p. injections of supraphysio-logical doses of coerulein (40 mg/kg bw) and necrotizing pancreatitis (NP) by retrograde injection of olive oil in the pancreatic duct (1 ml/kg bw). After 30 min, 3 h and 24 h respectively the pancreas was removed. Untreated animals and animals with saline injection served as controls. After whole organ pancreatic RNA-isolation RNA was pooled and hybridized to Affymetric chips to investigate 7000 genes and 1000 ESTs. We focused on genes with > 5-fold increase of expression activity in all pancreatitis groups compared to controls. Overall gene expression activity showed 17, 50 and 38 genes in CIP and 41, 106 and 240 genes in NP with > 5-fold expression activity after 30 min, 3 h and 24 h respec-tively compared to controls. 30 min after pancreatitis induction Krox-24, NGFI-A and -B, EGR-1 and p-jun-B were highly expressed. After 3 hours adrenodoxin, cyclophylin D and haemoxygenase G showed highest expression and after 24 hours cytochrome b558, the pancreatitis associated protein III, GTP-binging protein, EST AA 891596 were highly expressed. The heat shock protein 70 and immediate early serum protein and EST AA957003 were increasingly expressed in NP but not in CIP. Analysis of peak expression genes shows similar expression patterns during the initial phase in CIP and NP. Different expression is seen in only a few genes later in time course of disease and might reflect specific expression patterns in different forms of pancreatitis. |
