| Autor: |
Lethicia Danaelle Mafo Kamga, Raspail Carrel Founou, Michel Noubom, Brice Davy Dimani, Jessica Ravanola Zemtsa, Patrice Landry Koudoum, Aurelia Djeumako Mbossi, Thibau Flaurant Tchouangueu, Diomede Noukeu, Luria Leslie Founou, Donatien Gatsing |
| Rok vydání: |
2023 |
| Popis: |
Extended-spectrum β-lactamase (ESBL) mediating resistance in Enterobacterales is a global public health issue, especially in low-and middle-income countries (LMICs) such as Cameroon. ESBL-producing Enterobacterales reduce therapeutic options and lead to the use of last resort drugs such as carbapenems even in vulnerable populations like children under five years. This study aims at determining the phenotypic and genotypic characteristics of ESBL-producing Escherichia coli (ESBL-Ec) isolated from children under five years with and without diarrhoea in two health care facilities in Dschang. A cross-sectional study was conducted from 3 February to 19 May 2022 in two hospitals in the city of Dschang, Cameroon. Stool collected were cultured on Eosine Methylen Blue (EMB) medium. Enterosystem 18 R kit was used for bacterial identification. Evaluation of the resistance patterns and detection of ESBL production were performed with, the Kirby Bauer disk diffusion method and CHROMagar® ESBL medium, respectively. The genomic DNA of ESBL-Ec was extracted using the boiling method and subjected to conventional and multiplex PCRs for detection of blaSHV, blaCTX-M and blaTEM genes. Data were entered into ExcelTM 2016. Epi info and R software were used for statistical analyses with a p-value |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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