| Popis: |
The seed lectins of Phaseolus vulgaris cv. Contender typically consist of five tetrameric isolectins E4, E3L, E2L2, EL3 and L4. However electrophoretic and isoelectric focusing analysis of each purified protomer show a great heterogeneity. We demonstrate that each tetrameric isolectin exists in five different electrophoretic forms depending on the amount of Mn2+ cation bound to each isolectin. The effects of Mn2+ binding on charge and/or molecular properties of the protomers are discussed. Under the same experimental conditions, if we use Ca2+ ions instead of Mn2+, the abovemodifications are not observed. |
