Occurrence of grapevine leafroll-associated viruses-1 and -3 in Crimea
| Autor: | S. M. Gorislavets, E. V. Porotikova, Alexey A. Agranovsky, A. M. Kamionskaya, V. A. Volodin, E. P. Stranishevskaya, Y. A. Volkov, S.V. Vinogradova, V. I. Risovannaya, U. D. Dmitrenko |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Pcr cloning Biology 01 natural sciences General Biochemistry Genetics and Molecular Biology Virus 03 medical and health sciences 030104 developmental biology Specific primers Botany General Agricultural and Biological Sciences Vitis vinifera 010606 plant biology & botany General Environmental Science |
| Zdroj: | Moscow University Biological Sciences Bulletin. 71:76-79 |
| ISSN: | 1934-791X 0096-3925 |
| DOI: | 10.3103/s0096392516020097 |
| Popis: | Grapevine leafroll is one of the most widespread and harmful grapevine diseases. To investigate the occurrence of grapevine leafroll-associated viruses-1 and 3, the survey of vineyards has been conducted in 2015 in six regions of the Crimea. A total of 689 leaf samples with virus symptoms were collected. GLRaV-1 and GLRaV-3 were analyzed by RT-PCR with the specific primers, followed by sequencing of the PCR products. GLRaV-1 and GLRaV-3 were detected in 34 (4.9%) and 37 (5.4%) of the samples, respectively. Vineyards in Simferopol, Bakhchisarai, and Sevastopol regions were found to be free of GLRaV-1 and GLRaV-3. |
| Databáze: | OpenAIRE |
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