Fractional laser-assisted drug delivery: Active filling of laser channels with pressure and vacuum alteration
| Autor: | Merete Haedersdal, Andrés M. Erlendsson, R. Rox Anderson, William A. Farinelli, Apostolos G. Doukas, Brijesh Bhayana |
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| Rok vydání: | 2015 |
| Předmět: |
Biodistribution
Materials science Fractional laser Analytical chemistry 02 engineering and technology Dermatology Polyethylene glycol 021001 nanoscience & nanotechnology Laser Treatment efficacy Drug uptake law.invention 030207 dermatology & venereal diseases 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine chemistry law Drug delivery Surgery 0210 nano-technology Biomedical engineering Mathematical simulation |
| Zdroj: | Lasers in Surgery and Medicine. 48:116-124 |
| ISSN: | 0196-8092 |
| DOI: | 10.1002/lsm.22374 |
| Popis: | Background and Objective Ablative fractional laser (AFXL) is rapidly evolving as one of the foremost techniques for cutaneous drug delivery. While AFXL has effectively improved topical drug-induced clearance rates of actinic keratosis, treatment of basal cell carcinomas (BCCs) has been challenging, potentially due to insufficient drug uptake in deeper skin layers. This study sought to investigate a standardized method to actively fill laser-generated channels by altering pressure, vacuum, and pressure (PVP), enquiring its effect on (i) relative filling of individual laser channels; (ii) cutaneous deposition and delivery kinetics; (iii) biodistribution and diffusion pattern, estimated by mathematical simulation. Methods Franz diffusion chambers (FCs) were used to evaluate the PVP-technique, comparing passive (AFXL) and active (AFXL + PVP) channel filling. A fractional CO2-laser generated superficial (225 µm;17.5 mJ/channel) and deep (1200 µm; 130.5 mJ/channel) channels, and PVP was delivered as a 3-minutes cycle of 1 minute pressure (+1.0 atm), 1 minute vacuum (−1.0 atm), and 1 minute pressure (+1.0 atm). Filling of laser channels was visualized with a colored biomarker liquid (n = 12 FCs, n = 588 channels). Nuclear magnetic resonance quantified intracutaneous deposition of topically applied polyethylene glycol (PEG400) over time (10 minutes, 1 hour, and 4 hours), investigated with (n = 36 FCs) and without (n = 30 FCs) PVP-filling. Two-dimensional mathematical simulation was used to simulate intradermal biodistribution and diffusion at a depth of 1,000 µm. Results Active filling with application of PVP increased the number of filled laser channels. At a depth of 1,000 µm, filling increased from 44% (AFXL) to 94% with one PVP cycle (AFXL + PVP; P |
| Databáze: | OpenAIRE |
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