Effects of various pretreatments on seed germination ofCalystegia soldanella (Convolvulaceae), a coastal sand dune plant

Autor: Hye-Jeong Park, Hyeon-Cheol Cha, Byeong-Mee Min, Jung-Moon Ko
Rok vydání: 2004
Předmět:
Zdroj: Journal of Plant Biology. 47:396-400
ISSN: 1867-0725
1226-9239
Popis: We tested various pretreatments to enhance the germination of ‘Sea bells’ (Calystegia soldanella Roem. et Schult). One experiment, which improved the germination rate by 70%, involved first scarifying the seed coats, then immersing the seeds in 50 ppm GA3 for 24 h. A GA3-alone pretreatment did not increase germination. However, the most effective method, with a 100% success rate, included 3 h of acid pretreatment with 98% H2SO4. We also used scanning electron microscopy (SEM) to examine the seed-coat surfaces and cross-sections of dry seeds. SEM showed structural differences between seeds that were not treated and those exposed to 98% H2SO4. The latter treatment allowed the seed coats to crack and break, thereby disrupting their physical dormancy.
Databáze: OpenAIRE