Autor: Yves A. DeClerck, Alisa Lazaryev, R. Wajed, L.Q. Ma, Rex Moats, Nelson, Scott E. Fraser, Russell E. Jacobs, Yasuo Sugiura, M. Tyszka
Rok vydání: 2000
Předmět:
Zdroj: Clinical and Experimental Metastasis. 18:455-461
ISSN: 0262-0898
Popis: Neuroblastoma is the second most common solid tumor in children. So far few tumor models for this cancer have been reported in mice. We have created a murine tumor model for studying human neuroblastoma based on surgical orthotopic implantation in scid mice. Small fragments of subcutaneous tumors of SK-N-BE(2) human neuroblastoma cells expressing enhanced green fluorescent protein were surgically implanted near the left adrenal gland of scid mice. One hundred percent of the animals (n=21) successfully implanted developed a large retroperitoneal tumor and became moribund between 22 and 57 days after implantation (mean survival time = 41 days). At the time of sacrifice the presence of bone marrow metastasis was detected by RT-PCR for green fluorescent protein in 95% of the cases. The growth of small tumor implants could be easily visualized and quantified by surveillance MR imaging, with a resolution of 117×117×750 μm in two orthogonal planes allowing accurate volume measurements, as well as assessment of necrosis and tissue invasion. This novel model should be a valuable tool to study the biology and therapeutic approaches to neuroblastoma.
Databáze: OpenAIRE
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