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The successful translation of novel implantable technologies to clinical applications demands the comprehensive preclinical evaluation of the devices in vivo. This work aims to facilitate such translation by proposing a miniaturized multimodal thin-film deep brain stimulation ( $\mu$ DBS) device, tailored to rodent anatomy. The device was realized by incorporating glassy carbon (GC) electrodes into a thin-film polyimide-based substrate. The finished probe featured nine GC active sites with diameters ranging from $50\ \mu \mathrm{m}$ to $300\ \mu \mathrm{m}$ . The performed fast scan cyclic voltammetry revealed a dopamine sensitivity of $12.63 \pm 2.19\ \text{nA}\ \mu \mathrm{M}^{-1}, 20.72\pm 4.33\ \text{nA}\ \mu \mathrm{M}^{-1}, 135.44+8.24\ \text{nA} \mu \mathrm{M}^{-1}$ , and $68.50 \pm 3.42\ \mu \mathrm{M}^{-1}$ for electrodes with diameter of $50\ \mu \mathrm{m}, 100\ \mu \mathrm{m}, 200\ \mu \mathrm{m}$ , and $300\ \mu\mathrm{m}$ , respectively. The in vitro characterization assessed the $\mu\text{DBS}$ probe's ability to address electrical stimulation - in addition to neurochemical sensing - in a single integrated device. Future evaluation of the $\mu$ -DBS probes as a multimodal platform in animal models remains to be the next step in this path. |
