Changes of Aortic Tissue MicroRNA/mRNA Expression in Patients with Long-Term Left Ventricular Assist Device
| Autor: | Miroslav Konarik, Ivan Netuka, Peter Ivak, D. Dlouha, D. Hlavacek, J. Pitha, Z. Tucanova |
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| Rok vydání: | 2021 |
| Předmět: |
Pulmonary and Respiratory Medicine
Heart transplantation Transplantation Pathology medicine.medical_specialty Messenger RNA business.industry medicine.medical_treatment equipment and supplies Pathophysiology Downregulation and upregulation Ventricular assist device Gene expression microRNA Medicine Surgery Cardiology and Cardiovascular Medicine business Homeostasis |
| Zdroj: | The Journal of Heart and Lung Transplantation. 40:S450 |
| ISSN: | 1053-2498 |
| DOI: | 10.1016/j.healun.2021.01.1251 |
| Popis: | Purpose Attenuation of physiological pulsatility after continuous flow left ventricular assist device (LVAD) implantation has been reported as a contributor to alteration of aortic wall morphology and function. MicroRNAs are non-coding RNAs that act to regulate translation of messenger RNA (mRNA) into proteins and are fine-tuners of gene expression in response to pathophysiological stimuli. Differences in gene expression (mRNA) and in post-transcriptional regulators (microRNAs) could reflect biological response to exposure to the low pulsatile environment. We hypothesized that pulsatility deficit caused by LVAD may influence miRNA/mRNA expression in aortic tissue. The aim of the study was to detect miRNA/mRNA associations in the aortic tissue during LVAD support. Methods Aortic samples were collected at the time of LVAD implant and at heart transplantation in 16 patients (mean age 49.6±16.8 years; 3 females) and examined for miRNA/mRNA profiling. QuantSeq 3′ mRNA-Sequencing was used to examine differences in gene expression. Using human miRNome panels a profile of 752 miRNAs was identified. Results A total of 277 mRNAs were dysregulated after LVAD support (padj Conclusion The study provides additional insight in pathophysiology of vascular changes after LVAD implantation. A significant regulation of mRNAs involved in ECM and collagen fibers organization in response to implantation of LVAD was observed, which may suggest infliction of ECM homeostasis resulting in changes of intrinsic properties of vascular wall and arterial stiffness. Downregulation of miRNAs involved in molecular processes related to immune response, could implicate dysregulation in inflammatory processes after LVAD implantation. |
| Databáze: | OpenAIRE |
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