Expression of Macrophage Migration Inhibitory Factor Transcript and Protein by First-Trimester Human Trophoblasts1
| Autor: | R Vatti, Felice Arcuri, A. Carducci, Sabrina Liberatori, Luana Paulesu, Marcella Cintorino |
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| Rok vydání: | 1999 |
| Předmět: |
medicine.medical_specialty
Messenger RNA animal diseases medicine.medical_treatment chemical and pharmacologic phenomena Cell Biology General Medicine Biology Proinflammatory cytokine Cell biology Blot Endocrinology Cytokine medicine.anatomical_structure Reproductive Medicine Internal medicine embryonic structures Gene expression otorhinolaryngologic diseases medicine Chorionic villi Macrophage migration inhibitory factor Cytotrophoblasts |
| Zdroj: | Biology of Reproduction. 60:1299-1303 |
| ISSN: | 1529-7268 0006-3363 |
| Popis: | Macrophage migration inhibitory factor (MIF) was originally identified for its capacity to inhibit the random migration of macrophages in vitro. To date, the role of MIF as a pro-inflammatory cytokine, pituitary hormone, and counter-regulator of glucocorticoid action on the immune response is commonly recognized. Although recent studies suggest an involvement of MIF in reproduction, no data exist on the expression of this cytokine in early human pregnancy. In this study, we evaluated the presence of MIF protein and mRNA in specimens of chorionic villi from first-trimester human placenta. Tissues were obtained at 6-10 wk of gestation and analyzed by Western blotting, reverse transcription-polymerase chain reaction, and immunohistochemistry. Our results demonstrate that human villous tissue is a novel site of MIF synthesis. In addition, immunohistochemical analysis identified MIF protein in the cytotrophoblasts of both the inner layer of villi and in the trophoblastic cell islands. We speculate that in view of its proinflammatory features, MIF might play a critical role in human implantation and in early embryonic development. |
| Databáze: | OpenAIRE |
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