| Popis: |
At present, the recurrent aphthous stomatitis (RAS) are not completely clear. Therefore, identifying the underlying diagnostic biomarkers of RAS can provide new ideas for the diagnosis and treatment of RAS. The gene chip data of RAS (GSE37265) were downloaded from the NCBI Gene Expression Omnibus (GEO) database. Weighted Gene Co-Expression Network Analysis (WGCNA) was used to construct a co-expression module. A total of 16 co-expression modules were identified, and 30 hub genes in the turquoise module were identified. In addition, functional analysis of hub genes in modules of interest was performed, which indicated that such hub genes were mainly involved in pathways related to immune response, virus infection, epithelial cell, signal transduction. module two clusters (highly interconnected regions) were determined in the network, with score=17.647 and 10, respectively, cluster 1 and cluster 2 are linked by STAT1 and ICAM1, it is speculated that STAT1 may be a primary gene of RAS. Finally, genistein, daidzein, kaempferol, resveratrol, rosmarinic acid, triptolide, quercetin and (-)-epigallocatechin-3-gallate were selected from the TCMSP database, and both of them is the STAT-1 inhibitor. The results of reverse molecular docking suggest that in addition to triptolide, (-)-Epigallocatechin-3-gallate and resveratrol, the other 5 compounds (flavonoids) with similar structures may bind to the same position of STAT1 protein with different docking score. In conclusion, our results screened potential biomarkers that might contribute to the diagnosis and treatment of RAS, STAT1 protein is one of the potential therapeutic targets of RAS, and this target can be used to screen potential compounds. |
