Evidence that Ha-Ras mediates two distinguishable intracellular signals activated by v-Src

Autor:	K. Alexandropoulos, S. A. Qureshi, U. R. Rapp, David A. Foster, J. T. Bruder, Myunghi Rim, C K Joseph
Rok vydání:	1992
Předmět:	Mutant v-Src Gene expression Cell Biology Biology Signal transduction Serum Response Element Protein kinase A Molecular Biology Biochemistry Molecular biology Protein kinase C Intracellular
Zdroj:	Journal of Biological Chemistry. 267:17635-17639
ISSN:	0021-9258
Popis:	v-Src activates promoters under the control of 12-O-tetradecanoylphorbol-13-acetate (TPA) response elements (TREs) and serum response elements (SREs) via two distinguishable intracellular signaling mechanisms. The induction of TRE- and SRE-mediated gene expression by v-Src could be distinguished by a differential sensitivity to depleting cells of protein kinase C (PKC) and to a dominant negative Raf-1 mutant. Thus, PKC depletion and the dominant negative Raf-1 mutant were able to distinguish two intracellular signaling mechanisms activated by v-Src. Both of these v-Src-induced intracellular signals were sensitive to a dominant negative mutant of Ha-Ras. These data suggest that Ha-Ras functions to coordinately regulate multiple intracellular signaling mechanisms activated by v-Src.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::de497a73bc33d7f1f30a19fe506256c8 https://doi.org/10.1016/s0021-9258(19)37090-5 Zobrazit plný text záznamu