Characterization and detection of cardiac Troponin-T protein by using ‘aptamer’ mediated biofunctionalization of ZnO thin-film transistor
| Autor: | Sandeep G. Surya, Dilip Kumar Agarwal, Manoj Kandpal |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Kelvin probe force microscope
Materials science Aptamer Cardiac marker technology industry and agriculture General Physics and Astronomy Nanotechnology 02 engineering and technology Surfaces and Interfaces General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences 0104 chemical sciences Surfaces Coatings and Films X-ray photoelectron spectroscopy Thin-film transistor Microscopy Thin film 0210 nano-technology Volta potential |
| Zdroj: | Applied Surface Science. 466:874-881 |
| ISSN: | 0169-4332 |
| DOI: | 10.1016/j.apsusc.2018.10.086 |
| Popis: | In this work, we have demonstrated the use of DNA aptamer as a selective bio recognizable entity towards the qualitative detection of cardiac Troponin-T (cTnT), a clinically important cardiac marker protein. Prior to this, the ZnO thin film surface as an active semiconducting channel material was first biofunctionalized with DNA aptamer by using standard biotin-streptavidin chemistry. Biofunctionalization was further confirmed by analyzing the ZnO surface with atomic force microscope (AFM) and X- ray photoelectron spectroscopy (XPS) techniques. In order to understand the efficacy of biomolecular interaction between cardiac Troponin-T (cTnT) and its corresponding aptamer, Kelvin Probe Force Microscopy (KPFM) technique was performed to monitor the effective contact potential difference. Further, to realize the essential technological importance of this methodology as a sensor platform, a thin-film transistor (TFT) micro device was fabricated using ZnO as a channel material and characterized. These fabricated devices were further used to demonstrate the qualitative detection of cardiac Troponin-T protein by employing aptamer-mediated biofunctionalization of ZnO TFT. |
| Databáze: | OpenAIRE |
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