| Popis: |
By using a bound spectral polarity-detecting label, we investigated the solvation power of the solvent near the polymeric matrix of some polar chromatographic media intended for the separation of biopolymers in aqueous solutions. It was found that with highly polar materials such as cellulose and cross-linked polydextran gels (Sepharose, Sephadex) the solvation power of water molecules is reduced and that the polarity detected in the vicinity of the sorbent is much lower. The chromatographic materials investigated (Sephadex derivatives for gel and ion-exchange chromatography, Sepharose derivatives for gel and hydrophobic chromatography, cellulose, Spheron, Dowex 1-X2 and ion-exchange materials based on cellulose, starch, Spheron and Glycophase-coated porous glass) were compared with respect to the magnitude of the difference between the polarity of the microenvironment of sorbents and the polarity of water. Effects that influence this difference are discussed. |
