Lesion of the maxillary gland of spirochete-infected brine shrimp: high-voltage electron microscopy of focal epithelial-cell injury
| Autor: | Min J. Song, Greta E. Tyson |
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| Rok vydání: | 1991 |
| Předmět: | |
| Zdroj: | Canadian Journal of Zoology. 69:1922-1930 |
| ISSN: | 1480-3283 0008-4301 |
| Popis: | High-voltage electron microscopy of serial sections (0.25 μm thick) was used to find and study lesions of the efferent tubule of the maxillary gland in a spirochete-infected brine shrimp (Artemia franciscana). The efferent-tubule lesion consists of a cluster of spirochetes and small vesicles situated in an indentation of the base of the epithelium and often involves discontinuity of the basal lamina. Of 12 lesions examined, 2 revealed obvious signs of focal epithelial-cell injury. In both cases a large vacuole was located in the epithelium adjacent to the basal cluster of spirochetes and appeared to be a damaged, swollen epithelial-cell process. In the larger of the two lesions the vacuole contained spirochetes, vesicles, altered mitochondria, and abnormal membranous configurations. It is postulated that host-cell injury was initiated by spirochetes entering a cellular process by direct penetration of the plasmalemma (i.e., by diacytosis). Observations of spirochetes inside a damaged process and intracytoplasmic spirochetes devoid of a host-derived membrane support the hypothesis that diacytosis occurred. It is also suggested that each lesion of the efferent tubule originated from one or more spirochetes that migrated from the hemolymph into the basal lamina and underwent replication to form a microcolony beneath the basal surface of the epithelium. |
| Databáze: | OpenAIRE |
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