Mutational Consequences on Replication of M13 Constructs Containing Isomeric Diol Epoxide Adducts in E. coli
| Autor: | Jane M. Sayer, Ingrid Pontén, Myron F. Goodman, Anthony Dipple, Leilani A. Ramos, Govind Kalena, Heiko Kroth, Haruhiko Yagi, Donald M. Jerina |
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| Rok vydání: | 2002 |
| Předmět: | |
| Zdroj: | Polycyclic Aromatic Compounds. 22:871-879 |
| ISSN: | 1563-5333 1040-6638 |
| DOI: | 10.1080/10406630290104022 |
| Popis: | Eight isomeric dGuo and eight dAdo adducts resulting from cis and trans opening of each of the four optically active diol epoxides (DEs) derived from benzo[ a ]pyrene (B a P) and benzo[ c ]phenanthrene (B c Ph) were placed in each of two 16-mer DNA sequences to give 32 modified oligonucleotides, which were ligated into M13mp7L2 and allowed to replicate in SOS-induced Escherichia coli . The effects of parent hydrocarbon, adduct stereochemistry, and sequence context on mutagenic response are highly interdependent. B a P DE adducts are generally more mutagenic than the corresponding B c Ph adducts. The mutational frequency is generally larger for cis - relative to trans -opened DE adducts of both dGuo and dAdo. In a ∼TA*G∼ context, B c Ph DE dAdo adducts (A*) with R configuration at the site of attachment to the adenine base produced very few substitution mutations when compared with adducts having S configuration. This configurational effect is not observed for B a P DE dAdo adducts, nor for B a P or B c Ph... |
| Databáze: | OpenAIRE |
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