HPLC-based measurement of the chelator 1,2-dimethyl-3-hydroxy-pyrid-4-one (L1) and its iron complex for pharmacokinetic studies in humans

Autor:	Bernd Dresow, Gritta Janka, Roland Fischer, Erich E. Gabbe
Rok vydání:	1995
Předmět:	Excretion Chromatography Pharmacokinetics Chemistry Urinary system Chelation Urine Reversed-phase chromatography Biochemistry High-performance liquid chromatography Quantitative analysis (chemistry) Analytical Chemistry
Zdroj:	Fresenius' Journal of Analytical Chemistry. 352:562-564
ISSN:	1618-2650 0937-0633
DOI:	10.1007/bf00323074
Popis:	An improved HPLC based method to assay the oral active iron chelator 1,2-dimethyl-3-hydroxypyrid-4-one (L1, CP20) in serum and urine is described. The L1 peak has been well separated from other endogenous compounds, allowing the exact determination of the drug in both biological fluids. Moreover urinary iron excretion due to L1 therapy has been monitored by measuring urine Fe-(L1)3 complex concentrations using reverse phase HPLC and subsequent detection at 450 nm. In patients and normal volunteers receiving this drug there is a good correlation between urine iron excretion measured by AAS and by the HPLC based method.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ddfa2ed7d7f326881d6b60445d044dbd https://doi.org/10.1007/bf00323074 Zobrazit plný text záznamu Full text from SpringerLink