Recent advances in electrochemiluminescence resonance energy transfer for bioanalysis: Fundamentals and applications

Autor: Hong-Yuan Chen, Hai-Jie Lu, Hong Zhou, Jing-Juan Xu
Rok vydání: 2020
Předmět:
Zdroj: TrAC Trends in Analytical Chemistry. 122:115746
ISSN: 0165-9936
DOI: 10.1016/j.trac.2019.115746
Popis: Electrochemiluminescence resonance energy transfer (ERET) is a powerful analytical strategy based on the resonance energy transfer (RET) between an efficient electrochemiluminescence (ECL) emitter and a spectrally matched energy acceptor. Compared with fluorescence resonance energy transfer (FRET), ERET is more intriguing because it can eliminate the interference from unselective photoexcitation and the scattered light. In recent years, ERET has drawn wide concern for the analysis of the trace amounts of different targets due to its remarkable advantages of high sensitivity, inexpensive instruments and excellent controllability. This review detailly discuss the main mechanisms for the ERET process between the donor-acceptor pairs. We also comment on the latest progress of signaling strategies in the ERET process and their applications for the bioanalysis of small molecules, proteins and nucleic acids. Finally, perspectives and challenges in this field are also discussed.
Databáze: OpenAIRE