DC-driven thermoelectric Peltier device for precise DNA amplification
| Autor: | Kazuhito Inoue, Shigeo Yamaguchi, Tadzunu Suzuki, Yoshitaka Azumi |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Materials science
Thermoelectric cooling Physics and Astronomy (miscellaneous) business.industry General Engineering General Physics and Astronomy Nanotechnology Thermoelectric materials law.invention genomic DNA chemistry.chemical_compound chemistry law Thermoelectric effect Optoelectronics A-DNA Electric current business Polymerase chain reaction DNA |
| Zdroj: | Japanese Journal of Applied Physics. 54:057001 |
| ISSN: | 1347-4065 0021-4922 |
| DOI: | 10.7567/jjap.54.057001 |
| Popis: | Using a DC-driven Peltier device, we fabricated a DNA amplification system [polymerase chain reaction (PCR) system] with the aim of increasing its speed and precision. The Peltier device had a well block sandwiched by Bi2Se0.37Te2.36 as an N-type thermoelectric material and Bi0.59Sb1.30Te3 as a P-type material. The well block was directly controlled by the electric current, leading to a high thermal response. Using the Peltier device with the well block, we performed thermal cycles of a PCR, and we demonstrated that our PCR system produces a smaller amount of nonspecific products for the genome DNA (gDNA) of Arabidopsis thaliana, leading to a more precise DNA amplification system. |
| Databáze: | OpenAIRE |
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