| Autor: |
Ian H. Munro, Gareth R. Jones, N.K Kurek, M.N. Yakimenko, G. E. Dobretsov, T. Syrejshchikova, David T. Clarke |
| Rok vydání: |
2000 |
| Předmět: |
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| Zdroj: |
Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment. 448:471-477 |
| ISSN: |
0168-9002 |
| DOI: |
10.1016/s0168-9002(99)00687-7 |
| Popis: |
Time-resolved spectroscopy on the SRS of the Daresbury Laboratory was used for the study of the human serum lipoproteins and human blood albumins with fluorescent probes K-37 and K-35, developed in Russia. The probe K-37 was found sensitive to the difference in dynamic properties of the lipid objects. Two sets of the parameters were used for the description of lipid dynamic structure: (1) time-resolved fluorescence spectra and (2) time-resolved fluorescence depolarization as a function of rotational mobility of lipid molecules. Each measured dynamic parameter reflected the monotonous changes of dynamic properties in the range: lipid spheres–very low density lipoproteins–low density lipoproteins–high density lipoproteins–phospholipid liposomes. The range is characterized by the increase of the ratio polar/ nonpolar lipids. Thus, time-resolved fluorescence could be used to detect some structural modifications in lipoproteins related to atherosclerosis and subsequent cardiovascular diseases development. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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