| Autor: |
Alexander Nemuchin, Sofya Lushchekina, Bella Grigorenko, Sergey Varfolomeev |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Organophosphorous Neurotoxins ISBN: 9785369020265 |
| DOI: |
10.29039/chapter_5e4132b5f22366.15634219 |
| Popis: |
The work is devoted to modeling the elementary stages of the hydrolysis reaction in the active site of enzymes belonging to the class of cholinesterases — acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). The study allowed to describe at the molecular level the effect of the polymorphic modification of BChE, causing serious physiolog ical consequences. Cholinesterase plays a crucial role in the human body. AChE is one of the key enzymes of the central nervous system, and BChE performs protective functions in the body. According to the results of calculations using the combined method of quantum and molecular mechanics (KM/MM), the mechanism of the hydrolysis of the native acetylcholine substrate in the AChE active center was detailed. For a series of ester substrates, a method for estimation of dependence of the enzyme reactivity on the structure of the substrate has been developed. The mechanism of hydrolysis of the muscle relaxant of succininylcholine BChE and the effect of the Asp70Gly polymorph on it were studied. Using various computer simulation methods, the stability of the enzyme-substrate complex of two enzyme variants with succinylcholine was studied. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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