Rapid Extraction of Pawpaw Juice with the Application of Locally Produced Pectic Enzymes fromSaccharomyces CerevisiaeATCC 51712
Autor: | V.P. Dzogbefia, David Djokoto, J. H. Oldham |
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Rok vydání: | 2006 |
Předmět: | |
Zdroj: | Food Biotechnology. 20:31-41 |
ISSN: | 1532-4249 0890-5436 |
Popis: | Pawpaw (Carica papaya) fruit is highly perishable; hence, conversion into a value-added product such as juice concentrate will be useful. Pawpaw fruit is, however, high in pectin; therefore, its juice extraction is difficult without an extraction aid such as the addition of pectic enzymes. Our laboratory has developed a simple enzymatic method that enables the extraction of pawpaw juice with considerable ease. Pectic enzymes were obtained from Saccharomyces cerevisiae (ATCC 52712) cultured in pawpaw juice for 6 days at room temperature (25–28°C) and centrifuged to obtain the enzyme in the supernatant. Addition of this enzyme (32 mg total protein) to 200 g pawpaw mash and incubation for 30 min resulted in significant (P < 0.05) reduction in the viscosity of the mash with corresponding increases in the flow rate of free-run juice (85%). Simultaneously, large increases in the volume of free-run juice (180%) over the controls were obtained. Since the overall increase in total juice volume was only 35% as comp... |
Databáze: | OpenAIRE |
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