Popis: |
Seeds of Pisum sativum L. (two cultivars) and Oryza sativa L. cv. Oeiras were soaked for 0, 12, 24, 48, 72 or 96 h and then tested for emergence from moist sand. Carbon dioxide evolution under anoxia was measured in seeds soaked for up to 48 h. Pioneer pea, which gave the highest rate of CO 2 N 2 evolution, showed a decline in CO 2 N 2 evolution with time. The other two seed types (Meteor pea and rice) showed an increase in CO 2 N 2 evolution with time to 48 h. Total anaerobic metabolism over the soaking period (measured by CO 2 evolution under N 2 ) gave a better correlation with seed viability after soaking injury than measurements at any point in time of seed soaking. High total CO 2 N 2 evolution is associated with seed damage. The measurement of total CO 2 N 2 release was made by fitting lines to CO 2 N 2 liberation against time and integration of the curve. |