Micro-computed tomography assessment of vertebral column defects in retinoic acid-Induced rat model of myelomeningocele
| Autor: | Barbara Krynska, Michael E. Selzer, Mary F. Barbe, Nancy Pleshko, Oleg Gordiienko, Radhika Adiga |
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| Rok vydání: | 2014 |
| Předmět: |
Embryology
Pathology medicine.medical_specialty Axial skeleton X-ray microtomography business.industry Spina bifida Ossification General Medicine Anatomy Spinal cord medicine.disease Vertebra medicine.anatomical_structure Pediatrics Perinatology and Child Health medicine medicine.symptom business Lumbosacral joint Vertebral column Developmental Biology |
| Zdroj: | Birth Defects Research Part A: Clinical and Molecular Teratology. 100:453-462 |
| ISSN: | 1542-0752 |
| Popis: | Background Myelomeningocele (MMC) is a common congenital malformation and the most severe form of spina bifida characterized by the protrusion of spinal cord and meninges through the spinal defect. Our objective was to improve the assessment of congenital vertebral defects in animal models of MMC using three-dimensional high resolution micro-computed tomography (micro-CT) imaging and quantitative digital analyses methods. Methods Lumbosacral MMC was induced in fetal rats by exposure of pregnant mothers at embryonic day 10 (E10) to all-trans retinoic acid, and rats were examined at term (embryonic day 22). The axial skeleton was examined in an MMC model for the first time using ex vivo micro-CT at 10 μm voxel resolution to allow high resolution two-dimensional and three-dimensional characterization of anomalies in lumbosacral vertebrae, and quantitative assessment of distances between dorsal vertebral arches in lumbosacral regions in MMC rats, compared with normal controls. Results We observed, in detail, skeletal defects in lumbosacral vertebra of MMC rats, including in the morphology of individual dorsal vertebral arches. Use of high resolution micro-CT has also enabled us to identify the delayed (nonfused) or absent ossification in vertebral bodies, increased fusion of adjacent lateral vertebral elements, and quantify the extent of dorsal arch widening. Distances between dorsal vertebral arches showed statistically significant increases from L5 through S4 in MMC rats, compared with normal controls. Conclusion High-resolution micro-CT combined with digital quantification methods is a powerful technique ideally suited for precise assessment of complex congenital skeletal abnormalities such as examined in this rodent model of MMC. Birth Defects Research (Part A) 100:453–462, 2014. © 2014 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
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