Common wheat genotypes with null-alleles at gliadin loci
| Autor: | N. O. Kozub, I. O. Sozinov, H. Ya. Bidnyk, N. A. Demianova, O. I. Sozinova, A. V. Karelov, S. I. Spivak, Ya. B. Blume |
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| Rok vydání: | 2022 |
| Zdroj: | Faktori eksperimental'noi evolucii organizmiv. 31:59-64 |
| ISSN: | 2415-3826 2219-3782 |
| DOI: | 10.7124/feeo.v31.1485 |
| Popis: | Aim. The study was aimed at identification and selection of winter common wheat genotypes with null-alleles at the gliadin loci Gli-B1 and Gli-D1. Methods. The search for spontaneous mutants with the absence of synthesis of gliadin blocks was made in winter common wheat hybrid material and cultivars. To identify mutations, APAG electrophoresis and SDS-electrophoresis of seed storage proteins were performed. Results. The frequency of spontaneous mutations resulting in the null-allele at the loci Gli-B1 and Gli-D1 in F2 was 0,1 % and 0,05 %, respectively, in the cross Odesska chervonokolosa × B-16. Via sowing the material with those mutations and marker selection, F6 lines were developed: the line OB-Bnull with the null-allele at the Gli-B1 locus and the line OB-Dnull with the null-allele at the Gli-D1 locus. Another line with the null-allele at Gli-B1 was produced from the cross B3 × D4 by marker selection, as well as a biotype with the null-allele at the Gli-D1 locus was selected from the cultivar Slaven. Conclusions. The developed lines with null-alleles at the gliadin locus Gli-B1 (without omega-5 gliadins) and Gli-D1 (without omega-1,2 gliadins) are initial material for hypoallergenic wheat breeding. |
| Databáze: | OpenAIRE |
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