Quantitative characterization of biofunctionalization layers by robust image analysis for biosensor applications
| Autor: | Denis Flandre, Numa Couniot, Laurent Francis, Laurent Jacques, N. Van Overstraeten-Schlogel, Jonathan Rasson |
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| Rok vydání: | 2016 |
| Předmět: |
Materials science
Image processing Nanotechnology 02 engineering and technology 01 natural sciences law.invention Robustness (computer science) Confocal microscopy law Materials Chemistry Electrical and Electronic Engineering Instrumentation chemistry.chemical_classification Reproducibility Biomolecule 010401 analytical chemistry Metals and Alloys 021001 nanoscience & nanotechnology Condensed Matter Physics Fluorescence 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Characterization (materials science) chemistry 0210 nano-technology Biosensor |
| Zdroj: | Sensors and Actuators B: Chemical. 222:980-986 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2015.09.028 |
| Popis: | This work describes the development of a characterization method for biofunctionalized surfaces and its use for biosensor applications. The method is based on the processing of fluorescence images obtained by confocal microscopy. It retrieves quantitative data about the quantity and distribution of surface bound biomolecules, used to improve a biosensor performances in terms of specificity and sensitivity. Two biofunctionalization protocols were compared using the developed tools based on the fluorescence intensity, the surface coverage and the homogeneity of repartition of the fluorescence. In addition, the stability of the layer under flow, as well as the reproducibility and robustness of the protocols were evaluated. |
| Databáze: | OpenAIRE |
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