Bacterial structural genomics target enabled by a recently discovered potent fungal acetyl-CoA synthetase inhibitor

Autor:	Nicholas D. DeBouver, Madison J. Bolejack, Taiwo E. Esan, Damian J. Krysan, Timothy J. Hagen, Jan Abendroth
Rok vydání:	2023
Předmět:	Structural Biology Genetics Biophysics Condensed Matter Physics Biochemistry
Zdroj:	Acta Crystallographica Section F Structural Biology Communications. 79:137-143
ISSN:	2053-230X
DOI:	10.1107/s2053230x23003801
Popis:	The compound ethyl-adenosyl monophosphate ester (ethyl-AMP) has been shown to effectively inhibit acetyl-CoA synthetase (ACS) enzymes and to facilitate the crystallization of fungal ACS enzymes in various contexts. In this study, the addition of ethyl-AMP to a bacterial ACS from Legionella pneumophila resulted in the determination of a co-crystal structure of this previously elusive structural genomics target. The dual functionality of ethyl-AMP in both inhibiting ACS enzymes and promoting crystallization establishes its significance as a valuable resource for advancing structural investigations of this class of proteins.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ddaf2bcf56d804e8430cfa56c5c44959 https://doi.org/10.1107/s2053230x23003801 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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