Development and validation of fingerprints ofTurnera diffusaextracts obtained by use of high-performance liquid chromatography with diode array detection and chemometric methods

Autor:	M. L. Salazar Cavazos, A. Garza-Juárez, N. Waksman-De-Torres, R. Ramírez-Durón
Rok vydání:	2009
Předmět:	Chromatography biology Chemistry Analytical chemistry General Chemistry biology.organism_classification High-performance liquid chromatography Diode array Turnera diffusa Chemometrics chemistry.chemical_compound Fingerprint Trifluoroacetic acid Retention time
Zdroj:	Acta Chromatographica. 21:217-235
ISSN:	2083-5736 1233-2356
DOI:	10.1556/achrom.21.2009.2.3
Popis:	Summary A high-performance liquid chromatographic method with diode-array detection (HPLC-DAD) has been developed and validated to establish the fingerprint of Turnera diffusa. Hydroalcoholic extracts were obtained from 19 raw herbal samples collected in different regions of Mexico. Separation was performed on a 150 mm × 3.9 mm (4-μm particle) C18 column, using a gradient of methanol and 0.1% aqueous trifluoroacetic acid as the mobile phase. Chromatograms were recorded at 254 nm. To identify each peak, both retention time and peak spectrum were used. Intraday and interday relative standard deviations were
Databáze:	OpenAIRE
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