Monogenic Dominant Sulfonylurea Resistance in Sugarbeet from Somatic Cell Selection

Autor: Karen A. Renner, George Acquaah, William P. Doley, Joseph W. Saunders
Rok vydání: 1992
Předmět:
Zdroj: Crop Science. 32:1357-1360
ISSN: 1435-0653
0011-183X
DOI: 10.2135/cropsci1992.0011183x003200060010x
Popis: Injury to sugarbeet, Beta vulgaris L., from sulfonylurea herbicide residues from preceding cropping years has kindled interest in developing resistant cultivars. This study was conducted to obtain chlorsulfuron (2-chloro-N-[[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl]benzenesulfonamide) resistance from cell cultures and to determine its inheritance and magnitude. Utilizing annual diploid sugarbeet clone REL-1, dispersed suspension cultures were initiated from callus induced on leaf disk cultured on a modified Murashige and Skoog (MS) agar medium + 1.0 mg L⁻¹ N⁶-benzyladenine (BA) and placed in the liquid form of the same medium. Unmutagenized cell clusters were plated on solid medium containing 2.8 nM chlorsulfuron in MS + 1.0 mg L⁻¹ BA. A single colony arose, from which shoots were regenerated. Shoots were resistant to 28 nM chlorsulfuron, a concentration that killed similar shoots of REL-1. Resistance (designated Sur) was inherited as a monogenic dominant. In vitro shoot resistance to chlorsulfuron was 300 to 1000-fold greater than in REL-1. Resistance was also expressed in leaf disk expansion in vitro with MS + 1.0 mg L⁻¹ BA. Acknowledgement is made to the Michigan Agricultural Experiment Station for its support of this research.
Databáze: OpenAIRE
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