3,6-dichloro-1,2,4,5-Tetrazine Assayed at High Doses in the Metastatic Breast Cancer Cell Line MDA-MB-231 Reduces Cell Numbers and Induces Apoptosis
| Autor: | Amel Ben Ammaar El Gaaied, Fatma Ben Aissa-Fennira, Refka Guermazi, Inès Limam, Khaoula Ben Younes, Mohamed Abdelkarim |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Chemistry Cell medicine.disease Metastatic breast cancer 03 medical and health sciences Tetrazine chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Cell culture Apoptosis 030220 oncology & carcinogenesis medicine Cancer research High doses General Pharmacology Toxicology and Pharmaceutics Mda mb 231 |
| Zdroj: | Current Bioactive Compounds. 16:546-550 |
| ISSN: | 1573-4072 |
| DOI: | 10.2174/1573407215666181224105826 |
| Popis: | Background: The lack of treatment options for patients with chemotherapy-resistant cancers is pushing the field toward the development of new therapies. 1,2,4,5-tetrazine derivatives are a class of heterocyclic compounds that exhibit a broad spectrum of antitumor activities. Objective: The purpose of this study was to assess the biological activity of four s-tetrazine derivatives by substitution of two chloride atom of 3,6-dichloro-1,2,4,5-tetrazine with long hydrophobic side chains. Methods: We analyzed the anti-proliferative effects of four s-tetrazine derivatives with MTT assay and their pro-apoptotic effect with AV/ IP flow cytometry analysis and Hoechst 33342 staining. Results: We demonstrated that 3,6-dichloro-1,2,4,5-tetrazine (compound (1)) has a cytotoxic effect and induces apoptosis. Conclusion: 3,6-dichloro-1,2,4,5-tetrazine presents a new cytotoxic drug against metastatic breast cancer cell line MDA-MB-231 in vitro. |
| Databáze: | OpenAIRE |
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