Characterization of Polymorphic Microsatellite Markers in Pinus armandii (Pinaceae), an Endemic Conifer Species to China
| Autor: | Xiao-Hao Yan, Chuan Niu, Zhong-Hu Li, Ruo-Nan Wang, Lin-Lin Gong, Wan-Lin Dong |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Population genetics Locus (genetics) Plant Science Pinus armandii Biology biology.organism_classification 010603 evolutionary biology 01 natural sciences Loss of heterozygosity 03 medical and health sciences 030104 developmental biology Pinaceae Botany Polymorphic Microsatellite Marker Microsatellite Allele Ecology Evolution Behavior and Systematics |
| Zdroj: | Applications in Plant Sciences. 4:1600072 |
| ISSN: | 2168-0450 |
| Popis: | Premise of the study: Pinus armandii (Pinaceae) is an important conifer tree species in central and southwestern China, and it plays a key role in the local forest ecosystems. To investigate its population genetics and design effective conservation strategies, we characterized 18 polymorphic microsatellite markers for this species. Methods and Results: Eighteen novel polymorphic and 16 monomorphic microsatellite loci of P. armandii were isolated using Illumina MiSeq technology. The number of alleles per locus ranged from two to five. The expected heterozygosity ranged from 0.061 to 0.609 with an average of 0.384, and the observed heterozygosity ranged from 0.063 to 0.947 with an average of 0.436. Seventeen loci could be successfully transferred to five related Pinus species (P. koraiensis, P. griffithii, P. sibirica, P. pumila, and P. bungeana). Conclusions: These novel microsatellites could potentially be used to investigate the population genetics of P. armandii and related species. |
| Databáze: | OpenAIRE |
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