Screening of Beta-Secretase Inhibitors by Capillary Electrophoresis-Mass Spectrometry
| Autor: | Roman Řemínek, Jan Schejbal, Zdeněk Glatz |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
chemistry.chemical_classification
Protease biology Chemistry medicine.medical_treatment 010401 analytical chemistry Mass spectrometry 01 natural sciences Capillary electrophoresis–mass spectrometry 3. Good health 0104 chemical sciences 03 medical and health sciences 0302 clinical medicine Enzyme Capillary electrophoresis Biochemistry Aspartic acid Amyloid precursor protein biology.protein medicine Amyloid precursor protein secretase 030217 neurology & neurosurgery |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071601624 |
| Popis: | Alzheimer's disease is the most common cause of dementia, currently afflicting almost 40 million patients worldwide. According to the amyloid cascade hypothesis, the pathogenesis of the disease could be slowed down or even stopped by the inhibition of beta-secretase, making this aspartic acid protease a potentially important drug target site. Capillary electrophoresis is a promising technique for screening putative enzyme inhibitors due to highly effective separations, minuscule sample and other chemicals consumption, compatibility with a variety of detection techniques, and high throughput via automation. This chapter presents a method based on capillary electrophoresis coupled to mass spectrometry detection for kinetic and inhibition assays of the beta-secretase reaction with a decapeptide derived from an amyloid precursor protein. |
| Databáze: | OpenAIRE |
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