Comparative Expression of E-cadherin, .ALPHA. and .BETA. Catenin in Salivary Gland Tumors
| Autor: | Wataru Kudeken, Kazuto Yamada, Kouji Tsukitani, Miyako Namba, Masahiko Mori, Paul M. Speight, Lian Jia Yang, Yoshiaki Takai |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Pathology
medicine.medical_specialty Histology Salivary gland Physiology Adenoid cystic carcinoma Myoepithelial cell Cell Biology Biology medicine.disease Biochemistry Pathology and Forensic Medicine Benign tumor Staining Pleomorphic adenoma Papillary adenocarcinoma medicine.anatomical_structure Catenin medicine |
| Zdroj: | ACTA HISTOCHEMICA ET CYTOCHEMICA. 32:305-313 |
| ISSN: | 1347-5800 0044-5991 |
| DOI: | 10.1267/ahc.32.305 |
| Popis: | To study the expression and role of adhesion molecules in normal salivary glands and their tumors, we have studied simultaneously the expression of E-cadherin, and associated cytoplasmic proteins, α-catenin and β-catenin. Paraffin sections were evaluated using the streptavidin biotin peroxidase complex (SBPC) method for E-cadherin, and the SBPC method with tyramide signal amplification (TCA) for α- and β-catenin.Normal submandibular (n=10), parotid (n=4) and oral minor glands (n=4) showed cell membrane staining for E-cadherin and a positive reaction in intercalated and striated ducts for α - and β -catenin. Pleomorphic adenoma (n = 17) showed cell membrane staining for E-cadherin and α-catenin in luminal and non-luminal cells, but were negative for β-catenin. E-cadherin was also focally or irregularly positive in modified myoepithelial cells. Warthin's tumors (n = 4) expressed Ecadherin only and were negative for a- and β-catenins. Papillary adenocarcinoma (n = 4) showed predominant staining for E-cadherin, with no expression in the other tumor cells. Adenoid cystic carcinoma (n = 4) showed only cell membrane positivity for E-cadherin, with an absence of α- and β-catenins in the luminal cells. Non-luminal tumor cells of adenoid cystic carcinoma were negative for E-cadherin and positive for α- and β-catenins. The results were concluded as follows, 1) there was uniform cell membrane staining for E-cadherin in normal and benign tumor epithelium, but α- and β-catenins showed different expression and heterogeneous localization, 2) loss of E-cadherin reactivity was seen in malignant lesions, 3) there was no correlation between the distribution of Ecadherin, α-catenin and β-catenin in benign or malignant tumors of salivary gland origins. The results show variability and heterogeneous expression for E-cadherin, α- and β-catenins in salivary gland tumors. |
| Databáze: | OpenAIRE |
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