Simplified heavy metal staining techniques demonstrated with Fast Plant leaf tissue

Autor:	Thomas G. Guilliams, Joseph B. Harris, Jeffery A. Schultz
Rok vydání:	1992
Předmět:	inorganic chemicals Chromatography food and beverages chemistry.chemical_element Cell Biology Matrix (biology) Biology Stain Staining Metal chemistry.chemical_compound chemistry Cytoplasm visual_art Brassica rapa Botany visual_art.visual_art_medium Osmium Phosphotungstic acid Molecular Biology
Zdroj:	Cell Research. 2:89-96
ISSN:	1748-7838 1001-0602
DOI:	10.1038/cr.1992.9
Popis:	Fast Plant (Brassica rapa, Cruciferae) leaf tissue fixed in glutaraldehyde-acrolein and post-fixed in osmium, was examined for response to several easily-prepared heavy metal stains. Lead and uranium, separately and in combination, gave typical results across the spectrum of cell organelles. As a single stain following osmium, bismuth produced images seemingly equivalent to lead and uranium. Phosphotungstic acid produced very good membrane delineation but produced a washed-out background image similar to that from lead staining. Carbohydrate compounds were especially responsive to ruthenium; the cytoplasm and the matrix of all organelles were also stained very well. The procedures were no more demanding than traditional staining methods and may be easily used in research and teaching. Fast Plant materials are a reliable, quick and easy source of living material.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::dd4a078827b1a6491f2b3e026f1f1b8f https://doi.org/10.1038/cr.1992.9 Zobrazit plný text záznamu