Imaging of wood tissue by ToF-SIMS: Critical evaluation and development of sample preparation techniques
| Autor: | H.-P Fagerholm, Pedro Fardim, Andrey Pranovich, Geoffrey Daniel, Elena N. Tokareva, Bjarne Holmbom |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Chemistry
Scanning electron microscope Metal ions in aqueous solution Extraction (chemistry) Analytical chemistry General Physics and Astronomy Surfaces and Interfaces General Chemistry Condensed Matter Physics Mass spectrometry Surfaces Coatings and Films Characterization (materials science) chemistry.chemical_compound Membrane Lignin Sample preparation |
| Zdroj: | Applied Surface Science. 253:7569-7577 |
| ISSN: | 0169-4332 |
| DOI: | 10.1016/j.apsusc.2007.03.059 |
| Popis: | Tissue of Norway spruce wood was investigated by time-of-flight secondary-ion mass spectrometry (ToF-SIMS) and field-emission scanning electron microscopy (FE-SEM). Different sample preparation techniques (including sectioning and drying) traditionally used for SEM analysis were compared and critically evaluated. A high contamination of wood surfaces by polytetrafluoroethylene (PTFE) introduced onto the sample during sectioning was detected by ToF-SIMS. A new protocol was developed and images of wood sections dried in different ways were compared. Main location of Mg, Na, K and Ca, but no lignin in membranes of bordered pits in spruce section surfaces was detected by ToF-SIMS imaging if critical point drying (CPD) or acetone extraction followed by drying under nitrogen flow (AEND) was applied. No specific locations of wood components as well as low signals from metal ions (Mg and Na) were observed on the wood surfaces of freeze-dried (FD) or air-dried (AD) sections. It was found that extractive substances, presenting on surfaces of FD and AD wood section, were evenly distributed on the surfaces and interfered with ToF-SIMS analysis. Our results indicated that ToF-SIMS imaging was critically sensitive to the sample preparation technique and a strict protocol for characterization of metals, lignin and polysaccharides in wood was suggested. |
| Databáze: | OpenAIRE |
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