Physiochemical and biological properties of modified collagen sponge from porcine skin

Autor: Jimin Wu, Yuanyuan Xu, Xizheng Zhang, Ruixin Li, Yong Guo, Jing Guan, Shujie Huang, Bo Ning, Pengfei Wang, Zhihong Li
Rok vydání: 2009
Předmět:
Zdroj: Journal of Wuhan University of Technology-Mater. Sci. Ed.. 24:619-626
ISSN: 1993-0437
1000-2413
DOI: 10.1007/s11595-009-4619-2
Popis: The aim of the present study was to compare one-step method to EDC/NHS crosslinking (EDC/NHS group) and one-step simultaneous method to EDC/NHS crosslinking and heparin immobilization (EDC/NHS- Heparin group) in improving physiochemical and biological properties of native collagen sponge (Control group). Modified collagen sponge overcome the disadvantages of native collagen sponge. IR spectra suggest the change of the functional groups. DSC data indicate that the stability of caloric transformation in EDC/NHS group is slightly higher than that of EDC/NHS-Heparin group. The crosslinking degree, stability against enzymes, stability in morphologically and biomechanical properties of EDC/NHS-Heparin group are higher than those of EDC/NHS group, whereas, the water-binding capacity in EDC/NHS-Heparin group is lower than that of EDC/NHS group. HUVECs in EDC/NHS-Heparin group scaffold proliferate fast, migrate well and distribute uniformly. One-step simultaneous method gains the better effects in above aspects, heparinized collagen matrices increase in angiogenic potential and suit for defect repairing and tissue engineering.
Databáze: OpenAIRE
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