Identification of the uridine 5'-diphosphoglucose (UDP-Glc) binding subunit of cellulose synthase in Acetobacter xylinum using the photoaffinity probe 5-azido-UDP-Glc

Autor:	Fong Chyr Lin, R. R. Drake, B. E. Haley, R. M. Brown
Rok vydání:	1990
Předmět:	chemistry.chemical_classification Photoaffinity labeling ATP synthase biology Protein subunit Cell Biology Biochemistry Uridine carbohydrates (lipids) chemistry.chemical_compound Enzyme chemistry biology.protein Enzyme kinetics Binding site Cellulose Molecular Biology
Zdroj:	Journal of Biological Chemistry. 265:4782-4784
ISSN:	0021-9258
DOI:	10.1016/s0021-9258(19)34039-6
Popis:	Photoaffinity labeling of purified cellulose synthase with [beta-32P]5-azidouridine 5'-diphosphoglucose (UDP-Glc) has been used to identify the UDP-Glc binding subunit of the cellulose synthase from Acetobacter xylinum strain ATCC 53582. The results showed exclusive labeling of an 83-kDa polypeptide. Photoinsertion of [beta-32P]5-azido-UDP-Glc is stimulated by the cellulose synthase activator, bis-(3'----5') cyclic diguanylic acid. Addition of increasing amounts of UDP-Glc prevents photolabeling of the 83-kDa polypeptide. The reversible and photocatalyzed binding of this photoprobe also showed saturation kinetics. These studies demonstrate that the 83-kDa polypeptide is the catalytic subunit of the cellulose synthase in A. xylinum strain ATCC 53582.
Databáze:	OpenAIRE
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