UDP-glucose:protein transglucosylase in developing maize endosperm
| Autor: | Juana S. Tandecarz, Andrea Rothschild |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
chemistry.chemical_classification
Glycosylation biology fungi food and beverages Fast protein liquid chromatography Plant Science General Medicine Endosperm carbohydrates (lipids) Glycogen phosphorylase chemistry.chemical_compound Enzyme chemistry Biochemistry Glycosyltransferase Genetics biology.protein Starch synthase Agronomy and Crop Science Glucan |
| Zdroj: | Plant Science. 97:119-127 |
| ISSN: | 0168-9452 |
| DOI: | 10.1016/0168-9452(94)90048-5 |
| Popis: | UDP-Glc:protein transglucosylase (UPTG) (EC 2.4.1.112), involved in the first step of a two-step mechanism proposed in potato tuber for protein-bound α-glucan synthesis, was found in developing maize endosperm. The enzyme transfers glucosyl residues from UDP-[14C]glucose to an endogenous protein acceptor in a Mn2+-dependent reaction in the absence of added primer. After SDS-polycrylamide gel electrophrresis of the reaction product, radioactivity co-migrated with a 38-kDa protein band. A two-step experiment shows that the developing endosperm of maize contains phosphorylase and starch synthase, capable of utilizing the UPTG reaction product formed during the first step as a primer for the synthesis of protein-bound glucan in the presence of the respective glucosyl donors during the second incubation step. |
| Databáze: | OpenAIRE |
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